Journal: Oncotarget

Article Title: MicroRNA-30a increases tight junction protein expression to suppress the epithelial-mesenchymal transition and metastasis by targeting Slug in breast cancer

doi: 10.18632/oncotarget.7656

Figure Lengend Snippet: ( A ) The cellular phenotype of miR-30a low /Slug high /Fascin high /Claudin low correlates with poor clinicopathological features in breast cancer. Immunostaining results for paraffin-embedded breast cancer tissue samples of LNM-negative and early-stage tumors (stage I and IIa; columns a and b) and LNM-positive and advanced-stage tumors (stages III and IV; columns c and d). In addition, the T/N ratio for miR-30a was < 0.50 in columns c and d and was ≥ 0.50 in columns a and b. Clinicopathological features of the tumors were determined according to the sixth edition of the AJCC Cancer Staging Manual . ( B ) The tumor-suppressor role of miR-30a relative to its inhibition of EMT. The targeting of Slug mRNA by miR-30 results in downregulation of fascin and upregulation of the tight junction proteins CLDN-1, CLDN-2, and CLDN-3, which downregulates EMT and, ultimately, reduces the rate of breast cancer progression.

Article Snippet: Cells were incubated separately with mouse anti-CLDN-1 (Invitrogen) or rabbit anti-CLDN-2 or anti-CLDN-3 (Novus Biologicals).

Techniques: Immunostaining, Inhibition